Objectives: In root canal treatment, final root canal irrigation plays an essential role in removing smear layers, especially inorganic components, in order to achieve hermetic obturation of the root canal system. This study aimed to evaluate the root canal cleanliness, sealer penetration, and apical sealing ability of the root canal system following final irrigation using chelating agents, namely nano-chitosan, which was compared to EDTA and novel silver citrate (NSC).