Objective: Our knowledge of the links among gastrointestinal diseases, psychiatric disorders and family environment in adolescents is largely based on very limited data. Thus, this paper aims to examine the psychiatric symptom levels and family function areas in youths, who underwent endoscopy and gastric biopsy because of gastrointestinal complaints and compare their results with healthy controls. Method: Patients aged 12-18 who were scheduled to undergo diagnostic endoscopy at the Gastroenterology outpatient clinic of a Pediatric Hospital, and age and gender- matched healthy controls from the COVID-19 vaccination outpatient clinic of the same hospital have been referred to Child and Adolescent Psychiatry Unit. Adolescents who were determined to have clinically normal intelligence in the psychiatric evaluation conducted by a Child and Adolescent Psychiatry specialist, were enrolled. Revised-Child Anxiety and Depression Scale (R-CADS) was used to assess depressive and anxiety symptom levels and Family Assessment Device (FAD) to determine family functionality. Results: Worse general family functioning is positively correlated with higher anxiety levels and total internalizing scores in the study group. Family history of any medical disease was detected to be statistically significantly higher in the "endoscopic findings +" (with abnormal signs) group Discussion: Contrary to expectations, we did not find a significant difference between patients with gastrointestinal problems and healthy controls in terms of psychiatric symptom levels. However, it's worthwhile noting that higher Protection Factor Index (PFI) (academic achievement and socio-economic status) and better family functions predicted lower depression scores of patients with gastrointestinal problems.