PURPOSE Diffusion-weighted imaging (DWI) using single-shot echo-planar imaging (ss-EPI) is prone to artifacts, geometric distortion, and T2* blurring. Readout-segmented echo-planar imaging (rs-EPI) may improve image quality in the DWI of cervical cancer (CC). This study aimed to compare the image quality between rs-EPI and ss-EPI DWI in CC and to evaluate whether the apparent diffusion coefficient (ADC) values of ss-EPI (ssADC) and rs-EPI (rsADC) can differentiate the status of lymphovascular space invasion (LVSI) and lymph node metastasis (LNM). METHODS This prospective study included 69 patients with CC who underwent ss-EPI and rs-EPI DWI before surgery. Qualitative reader scores, signal-to-noise ratio (SNR), contrast-to-noise ratio (CNR), and ADC values derived from ss-EPI and rs-EPI were compared. The differences in ADC values were analyzed in patients who were (a) LNM-positive (LNM+, n = 17) and LNM-negative (LNM-, n = 52); (b) LVSI-positive (LVSI+, n = 33) and LVSI-negative (LVSI-, n = 36). RESULTS The rs-EPIs of CC had higher subjective image quality scores and a lower SNR than ss-EPI (all P < 0.001); no significant differences existed between rs-EPI and ss-EPI for either CNR or ADC (CNR, P = 0.313; ADC, P = 0.949; P > 0.05 for all). The rsADC and ssADC of the LNM+ group were substantially lower than those of the LNM- group (rsADC, P = 0.000; ssADC, P = 0.000; P < 0.001 for all); the areas under the receiver operating characteristic curve were 0.855 and 0.851, respectively. However, there were no differences in ADC values between the LVSI+ and LVSI- groups (rsADC, P = 0.271; ssADC, P = 0.200; P > 0.05 for all). CONCLUSION Over a similar scan time, rs-EPI improves the qualitative image quality of DWI significantly more than ss-EPI and has good diagnostic accuracy for LNM status in CC. However, neither could predict the LVSI status. CLINICAL SIGNIFICANCE Readout-segmented EPI improves the qualitative image quality of DWI and has good diagnostic accuracy for LNM status in CC, compared with conventional ss-EPI. It is more inclined to qualitative analysis of CC foci and provides a better scheme when choosing the DWI sequence scanning strategy for CC.