OBJECTIVE The evaluation of the angle between the renal artery and the aorta, as well as the determination of the distance of renal arteries from the aortic bifurcation, was carried out in fetuses without anomalies during the second-trimester ultrasound examination. METHODS Between 18+0 and 23+6 gestational weeks, 100 singleton fetuses without anomalies were evaluated. Demographic parameters and obstetric examination findings of the participants were recorded. During the ultrasound examination, the angles of the renal arteries were measured by determining the angle formed between the long axis of the right/left renal arteries and the long axis of the descending aorta, with the point of intersection located inferiorly (rRA-Ao°, lRA-Ao°). The location of the right/left renal arteries on the aorta and the distance from the midpoint of where the renal arteries originate from the aorta to the aortic bifurcation were also determined (rRA-Ab, lRA-Ab). Intra-observer and inter-observer evaluations were performed, and intraclass correlation analysis (ICC) analysis was conducted. RESULTS The rRA-Ao° remains constant with gestational weeks, while lRA-Ao° increases with gestational weeks (p = 0.746; p= 0.048, respectively). The rRA-Ab and lRA-Ab increase with gestational weeks (p < 0.001). Intraobserver and interobserver agreement for rRA-Ao° and lRA-Ao° were moderate (intraobserver ICC, 0.641; 0.643; interobserver ICC, 0.612; 0.624 respectively) and for rRA-Ab and lRA-Ab were good (intraobserver ICC, 0.823; 0.769, interobserver ICC, 0.760; 0.778 respectively). CONCLUSION During the second trimester fetal ultrasound examination, rRA-Ao° remains constant regardless of the gestational week, while lRA-Ao°, rRA-Ab and lRA-Ab increase with gestational age.