PURPOSE This study aimed to evaluate the validity of two artificial intelligence (AI)-based bone age assess- ment programs, BoneXpert and VUNO Med-Bone Age (VUNO), compared with manual assessments using the Greulich-Pyle method in Turkish children. METHODS This study included a cohort of 292 pediatric cases, ranging in age from 1 to 15 years with an equal gender and number distribution in each age group. Two radiologists, who were unaware of the bone age determined by AI, independently evaluated the bone age. The statistical study involved using the intraclass correlation coefficient (ICC) to measure the level of agreement between the manual and AI-based assessments. RESULTS The ICC coefficients for the agreement between the manual measurements of two radiologists indi- cate almost perfect agreement. When all cases, regardless of gender and age group, were analyzed, an almost perfect positive agreement was observed between the manual and software measure - ments. When bone age calculations were analyzed separately for boys and girls, no statistically significant differences were found between the two AI-based methods in any subgroup. For boys regardless of age, the ICCs were 0.995 for VUNO and 0.994 for BoneXpert (z = 1.597, P = 0.110), while for girls, the ICCs were 0.994 and 0.995, respectively (z = -1.303, P = 0.193). The overall agreement with manual measurements was high for both VUNO and BoneXpert. In both boys and girls, the agreement remained consistent across different age groups. These findings indicate that both AI- based bone age assessment tools have a high degree of agreement with manual measurements across all age and gender groups, with no significant superiority of one method over the other. CONCLUSION Both BoneXpert and VUNO demonstrated high validity in assessing bone age, with no statistically significant differences between the two methods across gender or pubertal status groups. Notably, this study represents the first evaluation of both BoneXpert and VUNO for bone age assessment in Turkish children, highlighting their potential as reliable and clinically relevant tools for this popu- lation. CLINICAL SIGNIFICANCE Investigating the most suitable AI program for the Turkish population could be clinically significant.