Background Neuroblastomas are extracranial solid tumors caused by the differentiation and uncontrolled proliferation of immature sympathetic nervous system cells. This study investigated the effects of the anticarcinogenic cisplatin (Cis) on IKK-b, NF-kb, p53, Ki-67 protein, and gene expression in neuroblastoma cells. Materials and Methods SH-SY5Y cells were treated for 48 hours with various doses of Cis (1, 3 and 10 µM). IKBK1, MKI67, TP53, and NFKB1 gene expression analyses were completed by transcription-quantitative polymerase chain reaction (RT-qPCR). The levels of IKK-b, NF-kb, p53, and Ki-67 proteins were detected by Western blotting. RESULTS IKBKB gene expression was significantly increased at 1 and 3 µM and reduced significantly 10 µM compared to the control. NFKB1 gene expression significantly increased at 1 µM Cis, whereas it decreased when Cis concentration increased. TP53 gene expression significantly increased at 1 and 3 µM. MKI67 gene expression decreased with an increase in Cis concentration. In the low concentration (1 µM) of Cis group, while there was no significant change in IKK-b and p53 protein expressions, NF-kb expression significantly increased. However, in the groups administered with high-dose Cis (3 µM, 10 µM), where Cis showed a significant antiproliferative effect, IKK-b expressions decreased significantly, while NF-kb and p53 expression increased. The expression of the proliferation marker Ki-67 was also reduced in this group. CONCLUSION These results highlight the antiproliferative activity of Cis and its related NF-kb/IKK-b, p53, and Ki-67 proteins and the marked changes in gene expression in neuroblastoma cells.