Objective: Individuals with multiple sclerosis (MS) often exhibit reduced levels of physical activity (PA), which are influenced by psychological factors, including depression and perceptions of exercise. Accordingly, this study aimed to assess PA levels among individuals with MS and to examine the associations between depression, exercise perception, and PA levels. Materials and Methods: A total of 80 individuals with MS (mean age: 32.71±9.22 years) participated in this cross-sectional study. Depression was evaluated using the Beck Depression Inventory, exercise perception was measured with the Exercise Benefits/Barriers Scale, and PA levels was determined through the International Physical Activity Questionnaire-Short Form. Results: The majority of participants were classified as minimally active (72.5%), whereas 8.8% were inactive and 18.8% were very active. A weak but positive correlation was identified between depression and exercise barriers (r=0.443, p<0.001), as well as between depression and body mass index (r=0.314, p=0.005). No significant correlation was observed between depression and total PA level. Participants most frequently cited physical effort and environmental limitations as major barriers to exercise. Conclusion: Although most individuals with MS acknowledge the advantages of exercise, depression, and perceived barriers can impede their participation in PA. Addressing both psychological and environmental factors may enhance exercise adherence and overall disease management in this population.