Objectives: This study was carried out to establish the effect of sexual training for pregnant women on the attitude toward sexuality during pregnancy and pregnancy sexual response. Materials and Methods: This randomized controlled study was completed with 73 pregnant women (intervention group =36, control group =37). The data were collected using the Pregnancy Sexual Response Inventory (PSRI) and the Attitude Scale toward Sexuality during Pregnancy (AStSdP). Participants between the 14th and 22nd weeks of their pregnancy in the intervention group were provided with individual education which lasted two hours, on sexuality during pregnancy. Results: PSRI and AStSdP scores of both intervention and control groups resembled each other at the beginning of the study (p>0.05). The evaluation carried out four weeks later demonstrated that score averages for PSRI, AStSdP, anxiety about sexual intercourse during pregnancy, and approving sexuality during pregnancy of the intervention group were higher than the same scores for the control group (p<0.05). Conclusion: Sexual training during pregnancy positively affects attitudes toward sexuality and sexual response during pregnancy. Education of all pregnant women in the prenatal period about sexuality provided by nurses and midwives is recommended.