Research findings suggest that negative air ions have the potential to ameliorate allergies, anxiety, attention, abnormal behavior, cognition, depression, energy levels, fatigue, mood, ovarian cancer, performance, respiratory function, sleep quality, spasticity, and numerous other bodily functions by fostering alkalinity within the body. We conducted a first-phase clinical trial to assess the effects of negative air ions on individuals with cerebral palsy and polycystic ovary syndrome, aiming to determine if negative air ions intervention could improve these conditions.