INTRODUCTION: This study aimed to evaluate individuals’ sleep quality and anxiety levels in home isolation during the coronavirus disease 2019 (COVID-19) pandemic. METHODS: The study was planned in a descriptive cross-sectional design and was conducted between 01.07.2020–31.07.2020 with 186 individuals who were isolated at home due to contact with infected people and due to being infected with COVID-19. The subjects were asked to complete a sociodemographic data form, the Beck Anxiety Inventory and the Pittsburgh Sleep Quality Index. Results: The mean Pittsburg Sleep Quality Index was 5.33, and the number of participants with poor sleep quality was found to be 92 (49.46%). A significant correlation was found between sleep quality and anxiety scores (r=0.705; p<0.001). Female participants who were infected with COVID-19 had higher anxiety scores (mean Beck Anxiety Inventory score: 8.93) and worse sleep quality (mean Pittsburg Sleep Quality Index score: 6.11, p = 0.002). DISCUSSION AND CONCLUSION: The COVID-19 Pandemic affected anxiety and sleep quality in those isolated at home. This situation is more common among female participants and those confirmed as COVID-19 positive via RT-PCR (Reverse Transcription Polymerase Chain Reaction).